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Hydrolysis of cPrPMEDAP-Phe in the presence of SiHa cell extracts. (A) [14C]cPrPMEDAP-Phe (30 μM; 1.5 μCi/ml) was incubated in a buffer (pH 5.5) containing extract from SiHa cells (●), denatured extract (○), or buffer only (▾). The conversion to cPrPMEDAP was monitored by HLPC. (B) Effect of pH on the cPrPMEDAP-Phe-hydrolyzing activity present in SiHa extracts. The data are expressed as means ± standard deviations of two independent experiments.
