Table 1.
Activities of peptides against representative pathogens associated with CFa
| Peptide sequence | Neutrophil elastase concn (μg/ml) | MIC (μM) |
|
|---|---|---|---|
| P. aeruginosa | S. aureus | ||
| K-W-K-L-F-K-K-I-P-K-F-L-H-L-A-K-K-F-NH2 | 0 | 32 | 64 |
| E-E-A-A-A-K-W-K-L-F-K-K-I-P-K-F-L-H-L-A-K-K-F-NH2 | 0 | >128 | >128 |
| E-E-E-A-A-A-K-W-K-L-F-K-K-I-P-K-F-L-H-L-A-K-K-F-NH2 | 0 | >128 | >128 |
| E-E-E-E-A-A-A-K-W-K-L-F-K-K-I-P-K-F-L-H-L-A-K-K-F-NH2 | 0 | >128 | >128 |
| E-βA-E-E-E-E-A-A-A-K-W-K-L-F-K-K-I-P-K-F-L-H-L-A-K-K-F-NH2 | 0 | >128 | >128 |
| E-E-βA-E-E-E-E-A-A-A-K-W-K-L-F-K-K-I-P-K-F-L-H-L-A-K-K-F-NH2 | 0 | >128 | >128 |
| E-E-E-βA-E-E-E-E-A-A-A-K-W-K-L-F-K-K-I-P-K-F-L-H-L-A-K-K-F-NH2 | 0 | >128 | >128 |
| k-w-k-l-f-k-k-i-p-k-f-l-h-l-a-k-k-f-NH2 | 0 | 8 | 16 |
| k-w-k-l-f-k-k-i-p-k-f-l-h-l-a-k-k-f-NH2 | 0.15 | ND | 16 |
| Ac-E-E-E-E-A-A-A-k-w-k-l-f-k-k-i-p-k-f-l-h-l-a-k-k-f-NH2 | 0 | >128 | 128 |
| Ac-E-E-E-E-A-A-A-k-w-k-l-f-k-k-i-p-k-f-l-h-l-a-k-k-f-NH2 | 0.075 | 128 | 32 |
| Ac-E-E-E-E-A-A-A-k-w-k-l-f-k-k-i-p-k-f-l-h-l-a-k-k-f-NH2 | 0.15 | 16 | 16 |
| Ac-E-E-E-E-A-A-A-OH + k-w-k-l-f-k-k-i-p-k-f-l-h-l-a-k-k-f-NH2 | 0 | ND | 16 |
| A-A-k-w-k-l-f-k-k-i-p-k-f-l-h-l-a-k-k-f-NH2 | 0 | ND | 32 |
a
Lowercase letters denote d-amino acids; uppercase letters denote l-amino acids. βA, β-alanine spacer; Ac, N-terminal acetylation. P. aeruginosa strain PAO1 and S. aureus strain SH1000 were used. ND, not determined.