Fig. 3.

Calcein leakage from LUVs containing outer membrane components induced by 500 nM (A) and 250 nM (B) lacticin Q and containing lipid A or DPG induced by 500 nM (C) and 250 nM (D). Lacticin Q in buffer A (10 mM Tris, 75 mM NaCl, 1 mM EDTA, pH 7.0) was incubated at 30°C. EX and EM wavelengths were set at 490 and 520 nm, respectively. As controls, 0% leakage and 100% leakage were obtained by the addition of buffer and 0.1% Triton X-100, respectively. The final lipid concentration was 50 μM. The data are the average results from at least two experiments. It was possible to add 20% LPS or 10% lipid A to LUVs as a maximum ratio.