Table 2.
Lipids and LUVs used in this study
| Membrane and LUV component(s)a | Charge | Remarks |
|---|---|---|
| Lipids (abbreviation/source) | ||
| Egg yolk l-α-phosphatidylcholine (PC) | ± | A basal lipid |
| Egg yolk l-α-phosphatidyl-dl-glycerol (PG) | − | A main component of the bacterial membrane |
| Egg yolk l-α-phosphatidylethanolamine (PE) | ± | A main component of the inner leaflet of the outer membrane |
| Lipopolysaccharide (LPS; from Salmonella enterica serotype Minnesota Re 595) | − | A main component of the outer leaflet of the outer membrane |
| Lipid A (from Salmonella enterica serotype Minnesota Re 595) | − | A hydrophobic part of LPS |
| Diphosphatidylglycerol (DPG; from bovine heart) | − | A component of the bacterial membrane |
| LUVs (molar ratio) | ||
| PC-PG (5:5) | − | Basal LUVs |
| PE-PG (5:5) | − | PE-containing LUVs |
| PC-PG-LPS (5:3:2) | − | LPS-containing LUVs |
| PE-PG-LPS (5:3:2) | − | PE- and LPS-containing LUVs, similar to the outer membrane |
| PE-PG-lipid A (5:3:1) | − | PE- and lipid A-containing LUVs |
| PC-PG-DPG (5:3:2) | − | DPG-containing LUVs |
| PE-PG-DPG (5:3:2) | − | PE- and DPG-containing LUVs |
a
LUVs, large unilamellar vesicles.