Fig. 1.
Effect of antibiotic treatment on growth of C. difficile in the cecal contents of mice. Mice received daily subcutaneous antibiotic treatment for 5 days. At 6 h after the final antibiotic dose, the cecal contents were collected and inoculated with 104 CFU of the C. difficile strains/ml. Samples were incubated anaerobically for 24 h, and then serial dilutions were plated onto selective media for quantification of C. difficile and assayed for toxin production. If C. difficile was not detected, the lower limit of detection (∼2 log10 CFU/g) was assigned. Error bars represent standard errors.