Fig. 3.
Effect of subcutaneous antibiotic treatment on concentrations of total anaerobes in cecal contents of mice. Mice received daily subcutaneous antibiotic treatment for 5 days. At 6 h after the final antibiotic dose, the cecal contents were collected, transferred to an anaerobic chamber, and plated onto brucella agar to determine bacterial densities. Identical aliquots were plated onto brucella agar and incubated in room air to confirm than anaerobes were being measured. If organisms were not detected in stool, the lower limit of detection (4 log10 CFU/g) was assigned.