Fig. 4.

Fitness assays of azole-resistant and azole-susceptible isolates. (A) In vitro fitness assays. Strains BPY40 and BPY41 were inoculated in YEPD in single or mixed (1:1) cultures. Aliquots were taken from each culture in triplicate, and population ratios were calculated from the proportions of azole-susceptible and azole-resistant colonies plated onto YEPD agar as described in Materials and Methods. (B) In vivo fitness assays. Strains BPY40 and BPY41 were inoculated intravenously as single or mixed (1:1) cultures to three groups of immunocompetent mice as described in Materials and Methods. Kidneys were taken at given time points from sacrificed mice, and population ratios were measured as described above.