Table 2.
Properties and nature of CorA-resistant mutants of S. aureus SH1000 isolated in this study
| Strain | CorA MIC (μg/ml) | MyxB MIC (μg/ml) | Avg generation time (min) ± SD | Amino acid substitution (codon change)a |
|
|---|---|---|---|---|---|
| β subunit | β′ subunit | ||||
| SH1000 | 2 | 2 | 37.2 ± 3.1 | – | – |
| SH1000-Cor1 | >128 | >128 | 45.3 ± 3.1 | S1127P (TCA-CCA) | None |
| SH1000-Cor2 | 64 | >128 | 46.9 ± 3.6 | None | L1165R (CTT-CGT) |
| SH1000-Cor3 | >128 | >128 | 41.1 ± 4.9 | S1127L (TCA-TTA) | None |
| SH1000-Cor4 | >128 | >128 | 42.7 ± 2.2 | S1127L (TCA-TTA) | None |
| SH1000-Cor5 | 64 | >128 | 36.7 ± 3.7 | E1084K (GAA-AAA) | None |
| SH1000-Cor6 | >128 | >128 | 38.1 ± 4.4 | None | K334N (AAA-AAC) |
| SH1000-Cor7 | 128 | 128 | 44.5 ± 8.0 | L1131F (TTG-TTT) | None |
| SH1000-Cor8 | 64 | >128 | 47.8 ± 1.1 | None | L1165R (CTT-CGT) |
a
S. aureus numbering.