Fig. 3.
Effect of Mcm1 depletion on MDR1 overexpression mediated by hyperactive forms of Cap1 and Mrr1. The wild-type strain, SC5314; the conditional mcm1 mutant, MRcan42; the control strain, MRcan43; and independent derivatives (A and B) of MRcan42 and MRcan43 that were rendered homozygous for the CAP1ΔC333 or the MRR1P683S allele were grown for 4 h in the absence (light-gray bars) or presence (dark-gray bars) of doxycycline as described in Materials and Methods. MDR1 mRNA levels were determined by real-time RT-PCR and are presented as relative expression levels compared to those of the reference strain, SC5314, in the absence of doxycycline, which were set to 1. The graph shows the means and standard deviations of two independent experiments, with duplicate measurements performed with each strain. *, MDR1 expression levels in SC5314 and the parental strains MRcan42 and MRcan43 were too low to be visible in the graph.