Fig. 5.

Uptake of diamidines by T. b. brucei strain tbat1^−/−. Trypanosomes isolated from infected rats were immediately exposed to 10 μM permeant in complete HMI-9 media for up to 1 h at RT. Levels of accumulation of DB75 (a), DB820 (b), and CPD0801 (c) by tbat1^−/− (○) and wild-type s427 (■) are shown as percentages of the permeant concentration in s427 at 60 min. The data are representative of the results of at least three similar experiments, each performed in at least triplicate. Bars represent SEM of the triplicate determinations in this particular experiment; when not shown, error bars fall within the symbol.