Fig. 6.

Apparent kinetic parameters of diamidine uptake by trypanosomes. DB75 (a), DB820 (b), and CPD0801 (c) uptake rates were measured by immediately incubating s427 cells isolated from female Wistar rats for 10 min with 0, 0.01, 0.03, 0.1, 0.3, 1, 3, 10, or 30 μM permeant in complete HMI-9 media. The data are representative of the results of at least three similar experiments, each performed in at least triplicate. Bars represent SEM of the triplicate determinants in this particular experiment. When not shown, bars fall within the symbol. Apparent kinetic constants (averages ± SEM of the results of 3 or 4 independent experiments), determined by nonlinear regression using the Michaelis-Menten equation, are shown in Table 5.