Table 2.

Survival of cultures at 10 days after initiation of treatment^a

Drug	Concn	s427		tbat1−/−		B48
		Exposure time(s)	Survival ratio(s)	Exposure time(s)	Survival ratio(s)	Exposure time(s)	Survival ratio(s)
Diminazene	32 μM	5 min	0/3	3 h	0/3	NT	NT
		Pulse	1/3
	3.2 μM	3 h	0/3	6 h	2/3	NT	NT
	1.3 μMb	24 h	0/3	24 h	0/3	24 h	0/3
		6 h	1/3
	260 nMc	24 h	0/3	96 h	0/3	48 h	0/3
				48 h	1/3
	65 nMd	48 h	0/3	96 h	1/3	96 h	0/3
		24 h	1/3
	13 nMe	96 h	3/3	96 h	3/3	96 h	3/3
DB75	32 μM	Pulse	0/3	3 h	0/3	NT	NT
	3.2 μM	1 h	0/3	6 h	0/3	NT	NT
	150 nMb	24 h	0/3	48 h	0/3	48 h	0/3
	30 nMc	96 h	0/3	96 h	3/3	96 h	3/3
	7.5 nMd	96 h	3/3	96 h	3/3	96 h	3/3
	1.5 nMe	96 h	3/3	96 h	3/3	96 h	3/3
DB820	32 μM	5 min	0/3	6 h	0/3	NT	NT
				3 h	2/3
	3.2 μM	6 h	0/3	6 h	3/3	NT	NT
	1.5 μMb	24 h	0/3	24 h	0/3	24 h	0/3
		6 h	2/3
	300 nMc	24 h	0/3	96 h	0/3	96 h	0/3
	75 nMd	48 h	0/3	96 h	3/3	96 h	3/3
	15 nMe	96 h	3/3	96 h	3/3	96 h	3/3
CPD0801	32 μM	3 h	0/3	6 h	3/3	NT	NT
	3.2 μM	6 h	3/3	6 h	3/3	NT	NT
	13.6 μMb	24 h	0/3	24 h	0/3	24 h	0/3
	2.72 μMc	24 h	0/3	24 h	0/3	24 h	0/3
		6 h	2/3
	680 nMd	48 h	0/3	96 h	0/3	96 h	0/3
		24 h	2/3			48 h	2/3
	136 nMe	96 h	0/3	96 h	3/3	96 h	3/3

^aSurvival ratios represent surviving cultures of three replicate determinations at the end of the experiment. Exposures ranged from a “pulse” to 96 h for each drug concentration. The exposure values shown indicate the minimum incubation time necessary for trypanocidal effect. NT, not tested.

^b100 × IC₅₀ against wild-type s427 (alamarBlue assay).

^c20 × IC₅₀ against wild-type s427 (alamarBlue assay).

^d5 × IC₅₀ against wild-type s427 (alamarBlue assay).

^e1 × IC₅₀ against wild-type s427 (alamarBlue assay).