Table 1.

Strains and plasmids used or constructed

Strain or plasmid	Genotype/relevant characteristicsa	Reference or source
P. aeruginosa
PAO1	Reference strain completely sequenced	Laboratory collection
PAΔD	PAO1 ΔampD::lox	15
PAΔdB	PAO1 ΔdacB::lox	31
PAdacB	1A1 spontaneous dacB mutant (W273X) of PAO1	31
PAdacBΔD	PadacB ΔampD::lox	31
PAΔR	PAO1 ΔampR::lox	31
PAΔC	PAO1 ΔampC::lox	30
PAΔnZ	PAO1 ΔnagZ::lox	42
PAΔDnZ	PAO1 ΔampD::lox ΔnagZ::lox	42
PAΔdBnZ	PAO1 ΔdacB::lox ΔnagZ::lox	42
PAdacBΔDnZ	PadacB ΔampD::lox ΔnagZ::lox	42
PAΔG	PAO1 ΔampG::lox	This work
PAΔDG	PAO1 ΔampD::lox ΔampG::lox	This work
PAΔdBG	PAO1 ΔdacB::lox ΔampG::lox	This work
PAdacBΔDG	PadacB ΔampD::lox ΔampG::lox	This work
PAOD1	Spontaneous oprD null mutant (W65X) of PAO1	32
PAOD1ΔnZ	PAOD1 ΔnagZ::Gm lox	This work
PAOD1ΔG	PAOD1 ΔampG::Gm lox	This work
JSG2A1	Pan-β-lactam resistant P. aeruginosa clinical strain	14
JSG2A1ΔnZ	JSG2A1 ΔnagZ::Gm lox	This work
JSG2A1ΔG	JSG2A1 ΔampG::Gm lox	This work
MQB1C5	Pan-β-lactam-resistant P. aeruginosa clinical strain	14
MQB1C5ΔnZ	MQB1C5 ΔnagZ::Gm lox	This work
MQB1C5ΔG	MQB1C5 ΔampG::Gm lox	This work
OFC2I4	Pan-β-lactam-resistant P. aeruginosa clinical strain	14
OFC2I4ΔnZ	OFC2I4 ΔnagZ::Gm lox	This work
OFC2I4ΔG	OFC2I4 ΔampG::Gm lox	This work
E. coli
XL1-Blue	F′::Tn10 proA+proB+lacIq Δ(lacZ)M15/recA1 endA1 gyrA96 (Nalr) thi hsdR17 (rk− mk−) mcrB1	Laboratory collection
S17.1	recA pro (RP4-2Tet::Mu Kan::Tn7)	Laboratory collection
Plasmids
pUCP26	Tcr, pUC-18-based Escherichia-Pseudomonas shuttle vector	41
pUCP26ampG	pUC26 containing PAO1 ampG gene	This work
pEX100Tlink	AprsacB, pUC19-based gene replacement vector with an MCS	38
pEX18Tc	TcrsacB, pUC18-based gene replacement vector with an MCS	9
pUCGmlox	Apr Gmr, pUC18-based vector containing the lox-flanked aacC1 gene	38
pCM157	Tcr, cre expression vector	38
pEXnZ	pEX100Tlink containing 5′and 3′ flanking sequence of nagZ	42
pEXnZGm	pEX100Tlink containing 5′and 3′ flanking sequence of nagZ::Gm lox	42
pEXTcΔampG::Gm	pEX18Tc containing 5′and 3′ flanking sequence of ampG::Gm lox	This work

^aMCS, multiple cloning site; Ap^r, ampicillin resistant; Gm^r, gentamicin resistant; Tc^r, tetracycline resistant.