Table 2.
Primer | Sequence (5′–3′)a | PCR product size (bp) | Use |
---|---|---|---|
AGF1-ERI | GATATAGAATTCCGGTCGCGGCGGCACCATCTG | 823 | ampG inactivation |
AGR1-XbI | TATATCTCTAGAGCTGGCGGGAGACTTGTAGGC | ||
AGF2-XbI | GATATATCTAGATACGTCACCGCGGTGATGGGC | 621 | |
AGR2-HD3 | TATATCAAGCTTGTGCTGATCCTGCTGTTCCGC | ||
AGF-ERI | GATATAGAATTCAAGAAGGAGATATACATATGACTCAGCAATCCTGG | 1,785 | ampG cloning |
AGR-HD3 | TATATCAAGCTTTCAGTGCTGCTCGGCGTTCTGGTGTCCC |
Restriction sites for endonucleases are underlined.