Table 2.
Primers used for cloning and construction of ampG knockout mutants
| Primer | Sequence (5′–3′)a | PCR product size (bp) | Use |
|---|---|---|---|
| AGF1-ERI | GATATAGAATTCCGGTCGCGGCGGCACCATCTG | 823 | ampG inactivation |
| AGR1-XbI | TATATCTCTAGAGCTGGCGGGAGACTTGTAGGC | ||
| AGF2-XbI | GATATATCTAGATACGTCACCGCGGTGATGGGC | 621 | |
| AGR2-HD3 | TATATCAAGCTTGTGCTGATCCTGCTGTTCCGC | ||
| AGF-ERI | GATATAGAATTCAAGAAGGAGATATACATATGACTCAGCAATCCTGG | 1,785 | ampG cloning |
| AGR-HD3 | TATATCAAGCTTTCAGTGCTGCTCGGCGTTCTGGTGTCCC |
a
Restriction sites for endonucleases are underlined.