Table 1.
Principal oligonucleotide primer pairs used
| Procedure and genea | Primer | Sequence (5′–3′) | Source or reference | Product size (bp) |
|---|---|---|---|---|
| ICESp1108 mapping | ||||
| orf1 | ETR1 | GGGATAGGACTGATTGAA | This study | 10,110 |
| orf14 | ETR89 | GTGTTATACCTGTTGGAATACC | 6 | |
| orf14 | ETR99 | GGTATTCCAACAGGTATAACAC | 6 | 8,093 |
| orf20 | ETR94 | CTGTTCTTGGATATGTGATTAGC | 6 | |
| orf20 | ETR94-for | TTGGCTGGTAGGAATGAAT | This study | 6,991 |
| orf22 | ETR50-rev | TTTGAGTGGTAAGATGGTT | This study | |
| orf22 | ETR50 | TATGGTACAAGTAGAGTGAATGCC | 6 | 8,731 |
| erm(TR) | III8 | GCATGACATAAACCTTCA | 26 | |
| erm(TR) | TR1 | ATAGAAATTGGGTCAGGAAAAGG | 12 | 5,963 |
| orf34 | ETR13 | GCAAAAAAGCACGCAGAAAG | This study | |
| orf34 | ETR23 | GTCTTTCTGCTTGTAGTTCTGCC | This study | 5,369 |
| orf40 | ETR85 | GTTAGCAATAGTGTTTCTGTTTT | This study | |
| ICESp2905 mapping | ||||
| orf2 | CD1-for | AAAACGAACGGGAATATC | This study | 8,165 |
| erm(TR) | III8 | GCATGACATAAACCTTCA | 26 | |
| erm(TR) | TR1 | ATAGAAATTGGGTCAGGAAAAGG | 12 | 14,299 |
| orf29 | CD2-rev | TTGATGTAATGTAGGATAAAAG | This study | |
| orf29 | CD3-for | CAAAGCCAAATGTCCTAAATGAAA | This study | 13,452 |
| orf33 | CD3-rev | TCCGCAAAATCCGTCCTACA | This study | |
| orf34 | TnpV-for | GAATGGACAGGATGCCTCAA | This study | 14,319 |
| orf48 | CD4-rev | ACTCAAAATAATCGCTGTCCTT | This study | |
| orf49 | CD5-for | ATGTAGTTGAAAGAGAAAATAAGAA | This study | 12,981 |
| orf61 | CD5-rev | GGAGATTACCATACCGCCAACACC | This study | |
| ICESp1108 chromosomal integration site | ||||
| Spy1198* | LYT-for | TTACAGGGTCTGCGGCTATT | This study | 6,193 |
| orf5 | ETR49 | TCTTAGCTGGTATATACACTTACC | 6 | |
| orf37 | ETR45 | TCAAAAGCAGCATCTTTATCTTCG | 6 | 5,147 |
| Spy1195* | THIO-rev | TTCAAAGCCGTCTCAGTCAC | This study | |
| ICESp2905 chromosomal integration site | ||||
| Spy1099† | LYT-for | TTACAGGGTCTGCGGCTATT | This study | 2,043 |
| orf1 | TR-inv1 | CTAACTAAGAATCCAATAATCA | This study | |
| orf61 | CD6-for | TAATGTTGCGGTATTTTTTGA | This study | 2,445 |
| Spy1096† | THIO-rev | TTCAAAGCCGTCTCAGTCAC | This study | |
| Conserved core sequence | ||||
| erm(TR) | TR1 | ATAGAAATTGGGTCAGGAAAAGG | 12 | 1,497 |
| orf30‡ | Cyt-rev | ATGCCCTGAAGTTCCAAAG | This study | |
| Inverse PCR | ||||
| orf1‡ | C1LE-inv3 | TTCAATCAGTCCTATCCC | This study | |
| orf1‡ | C1LE-inv4 | AGTAAAGGGCAAAAGTCT | This study |
a
*, From the S. pyogenes MGAS10750 genome;
†
, from the S. pyogenes MGAS5005 genome;
‡
, from ICESp1108.