Table 2.
Oligonucleotide primers used in this study
| Oligonucleotide | Sequence (5′–3′)a |
|---|---|
| 467U | AAATATTTATAATGCAGTTAACG |
| 467L | GACCAGAAGTATAAACCATACCA |
| 2955U | ACACATTAGCTGTTAACATGACG |
| 2955L | ATGTTTCACTTCTAGCAACATGG |
| 467attB1F1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTAACTGCACTATGTACTGGAAGAGGA |
| 467attB2R1 | GGGGACCACTTTGTACAAGAAAGCTGGGTGAAGCTTGATTAAACATATTAATCG |
| 2955attB1F1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTTTAGATGAAGCAAAAGTCGTGTATC |
| 2955attB2R3 | GGGGACCACTTTGTACAAGAAAGCTGGGTATAAATTCATTTGATTAAGTGCTGT |
| 467_F1 | AAGAGTATTGGCATTTGAAATTGAT |
| 467_R1 | CCTTCTCAGCCTGACTAAGCAT |
| 467Sb | GATCCAAGACGTCGCGGTGAA |
| 467Sc | GAGTGTTGGGCATTATGTTCAATAC |
| 467Sd | CGAGTTTGGAAGAATTGGCTCTACT |
| 467Se | AACAGGCGAAGTCTTCAATAAGTGA |
| 467Sf | ACGTAGAACCTGGCAACAGTTTATC |
| 2955_F1 | TTCCATTTCAGACATAACGCCTGT |
| 2955_R1 | TGCATTAACCGAACCCTGTATATCA |
| 2955Sb | CACCGATGGCACCATTATATGTC |
| 2955Sc | GAAAGTTCAAATCAAGAGCCGTCA |
| 2955Sd | AAATGTTGGGAATCAGAAAACTTCA |
| 2955Se | CATTATGAACCTTGTCATCACGAAG |
| 2955Sf | CAAAGGGTACATCAGTTGGCAGTAC |
| mprFF1 | AAGAAGCACTCATAATCGGCTGTT |
| mprFR1 | GGCGACTTAACTTTAGCTCATTTCA |
| mprFS1 | TGTAGGTTTCGGTGGCTTTATTG |
| mprFS2 | CATCAGCTAAGAAGTACATTGAGGG |
| mprFS3 | GTATTGCGCTATTACTTCTGGCTTA |
| mprFS4 | TAACGCAATTTTCAACTTCAGGTAA |
a
Engineered attB sites for recombination of PCR amplicons into the pKOR1 vector are underlined.