Table 2.
Oligonucleotide | Sequence (5′–3′)a |
---|---|
467U | AAATATTTATAATGCAGTTAACG |
467L | GACCAGAAGTATAAACCATACCA |
2955U | ACACATTAGCTGTTAACATGACG |
2955L | ATGTTTCACTTCTAGCAACATGG |
467attB1F1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTAACTGCACTATGTACTGGAAGAGGA |
467attB2R1 | GGGGACCACTTTGTACAAGAAAGCTGGGTGAAGCTTGATTAAACATATTAATCG |
2955attB1F1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTTTAGATGAAGCAAAAGTCGTGTATC |
2955attB2R3 | GGGGACCACTTTGTACAAGAAAGCTGGGTATAAATTCATTTGATTAAGTGCTGT |
467_F1 | AAGAGTATTGGCATTTGAAATTGAT |
467_R1 | CCTTCTCAGCCTGACTAAGCAT |
467Sb | GATCCAAGACGTCGCGGTGAA |
467Sc | GAGTGTTGGGCATTATGTTCAATAC |
467Sd | CGAGTTTGGAAGAATTGGCTCTACT |
467Se | AACAGGCGAAGTCTTCAATAAGTGA |
467Sf | ACGTAGAACCTGGCAACAGTTTATC |
2955_F1 | TTCCATTTCAGACATAACGCCTGT |
2955_R1 | TGCATTAACCGAACCCTGTATATCA |
2955Sb | CACCGATGGCACCATTATATGTC |
2955Sc | GAAAGTTCAAATCAAGAGCCGTCA |
2955Sd | AAATGTTGGGAATCAGAAAACTTCA |
2955Se | CATTATGAACCTTGTCATCACGAAG |
2955Sf | CAAAGGGTACATCAGTTGGCAGTAC |
mprFF1 | AAGAAGCACTCATAATCGGCTGTT |
mprFR1 | GGCGACTTAACTTTAGCTCATTTCA |
mprFS1 | TGTAGGTTTCGGTGGCTTTATTG |
mprFS2 | CATCAGCTAAGAAGTACATTGAGGG |
mprFS3 | GTATTGCGCTATTACTTCTGGCTTA |
mprFS4 | TAACGCAATTTTCAACTTCAGGTAA |
Engineered attB sites for recombination of PCR amplicons into the pKOR1 vector are underlined.