Table 4.

DNA microarray^a hybridization profiles of the ACME arc-positive ST8-MRSA-IVa, ST22-MRSA-IVh, and ST22-MRSA-IVa isolates identified in the present study

Gene class	Gene(s)	Result for the following genotype/DNA microarray type of the indicated groupb (no. of isolates):
		ST8-MRSA-IVa/ST8-MRSA-IV			ST22-MRSA-IVa/ST22-MRSA-IV (a) (1)	ST22-MRSA-IVh/ST22-MRSA-IV
		(a) (5)	(b) (1)	(c) (1)		(b) (12)	(c) (1)	(d) (2)
Species markers	katA, coa, nuc, spa	Pos	Pos	Pos	Pos	Pos	Pos	Pos
agr group	agr group I	Pos	Pos	Pos	Pos	Pos	Pos	Pos
SCCmec-associated	mecA	Pos	Pos	Pos	Pos	Pos	Pos	Pos
markers	ΔmecR	Pos	Pos	Pos	Pos	Pos	Pos	Pos
	mecR-mecI	Neg	Neg	Neg	Neg	Neg	Neg	Neg
	ccrA-1 and ccrB-1	Neg	Neg	Neg	Neg	Neg	Neg	Neg
	Q9XB68-dcs	Pos	Pos	Pos	Pos	Pos	Pos	Pos
	ccrA-2 and ccrB-2	Pos	Pos	Pos	Pos	Pos	Pos	Pos
	kdp-SCC locus	Neg	Neg	Neg	Neg	Neg	Neg	Neg
	ccrA-3 and ccrB-3	Neg	Neg	Neg	Neg	Neg	Neg	Neg
	ccrC, ccrA-4, and ccrB-4	Neg	Neg	Neg	Neg	Neg	Neg	Neg
Antimicrobial resistance	blaZ-blaI-blaR1	Pos	Pos	Pos	Pos	Pos	Neg	Pos
genes	erm(A), erm(B)	Neg	Neg	Neg	Neg	Neg	Neg	Neg
	erm(C)	Neg	Neg	Neg	Pos	Pos	Pos	Pos
	lnu(A)	Neg	Neg	Neg	Neg	Pos	Neg	Neg
	msr(A), mph(C)	Pos	Pos	Pos	Neg	Neg	Neg	Neg
	aacA-aphD	Neg	Neg	Neg	Neg	Pos	Neg	Neg
	aadD	Neg	Neg	Neg	Neg	Pos	Neg	Neg
	aphA3-sat	Pos	Pos	Neg	Neg	Neg	Neg	Neg
	dfrS1	Neg	Neg	Neg	Neg	Neg	Neg	Neg
	far1	Neg	Neg	Neg	Neg	Neg	Neg	Neg
	mupA	Neg	Neg	Neg	Neg	Pos	Neg	Neg
	tet(K)	Neg	Neg	Neg	Neg	Neg	Neg	Neg
	tet(M)	Neg	Neg	Neg	Neg	Neg	Neg	Neg
	cat	Neg	Neg	Neg	Neg	Neg	Neg	Neg
	cfr	Neg	Pos	Neg	Neg	Neg	Neg	Neg
	fexA	Neg	Pos	Neg	Neg	Neg	Neg	Neg
	fosB	Pos	Pos	Pos	Neg	Neg	Neg	Neg
Virulence-associated	tst1	Neg	Neg	Neg	Neg	Neg	Neg	Neg
genes	sea, seb, see, seh	Neg	Neg	Neg	Neg	Neg	Neg	Neg
	sec and sel, sed, sej, and ser	Neg	Neg	Neg	Neg	Neg	Neg	Neg
	seg, sei, sem, sen, seo, and seu	Neg	Neg	Neg	Pos	Posc	Pos	Pos
	sek and seq	Pos	Pos	Pos	Neg	Neg	Neg	Neg
	lukF-PV, lukS-PV	Pos	Pos	Pos	Pos	Neg	Neg	Neg
	sak, chp, and scn	Pos	Pos	Pos	Neg	Pos	Pos	Pos
	etA, etB, and etC	Neg	Neg	Neg	Neg	Neg	Neg	Neg
	edinA, edinB, and edinC	Neg	Neg	Neg	Neg	Neg	Neg	Neg
	arcA, arcB, arcC, and arcD	Pos	Pos	Pos	Pos	Pos	Pos	Pos
Capsule type	Capsule type 5	Pos	Pos	Pos	Pos	Pos	Pos	Pos
	Capsule type 8	Neg	Neg	Neg	Neg	Neg	Neg	Neg

^aThe StaphyType kit (Alere Technologies) was used for DNA microarray analysis. Full data sets are available upon request.

^bIsolates within each ST-SCCmec type were assigned to DNA microarray groups on the basis of the presence of a unique combination of virulence and/or antimicrobial resistance genes. The ST8-MRSA-IV and ST22-MRSA-IV isolates were differentiated into three groups and four groups, respectively (shown in Table 3). Pos, positive; Neg, negative.

^cOne isolate, M08/0122, in microarray group ST22-MRSA-IV (b) was positive for the enterotoxin gene cluster genes seg, sem, sen, seo, and seu/y but lacked sei.