Sequence alignment showing the C domains of MRSA PK and human PK isoforms. GenBank accession numbers are YP_041163.1 for MRSA252 (this study), NP_872270.1 for human M1 (hM1), NP_002645.3 for human muscle isozyme M2 (hM2), NP_000289.1 for human LR1 (hLR1), and NP_870986.1 for human LR2 (hLR2). Sequences were aligned by using ClustalW2 software (3). Amino acids in boldface type indicate effector binding sites: the binding sites of the sugar, 1-phosphate, and 6-phosphate moieties of FBP are indicated by closed circles, open squares, and closed squares, respectively. The poorly conserved residues are shaded in the MRSA PK sequence. Absolutely conserved, highly conserved, and poorly conserved residues are marked by asterisks, colons, and dots, respectively.