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SDS-PAGE of purified MRSA PK and human PK isoforms. Five-microgram aliquots of MRSA PK and human M1, M2, R, and L PK proteins purified to homogeneity through the Ni-NTA chromatography step, as described in Materials and Methods, were applied onto lanes 1 to 5, respectively. Low-molecular-weight (LMW) standards (Pharmacia Biotech) were applied as size markers. Proteins were stained with Coomassie brilliant blue dye.
