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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Surgery. 2010 Dec;148(6):1228–1236. doi: 10.1016/j.surg.2010.09.026

Figure 4. Inhibition of RET phosphorylation and total RET protein expression by WA in vivo.

Figure 4

In ex-vivo tumors removed from Nu/Nu mice with DRO81-1 xenograft injections of 5 million cells to the left posterior neck, prosurvival proteins were analyzed by Western Blot analysis and demonstrate in the WA-treated animals both inhibition of phospho-RET and total RET with beta-actin levels demonstrating equal protein loading in lanes. The dose of WA used in the in vivo model is equivalent to 3 μM in vitro dosing. Quantitative densiometry is shown below to compare protein expression as a normalized ratio to beta-actin levels.