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. Author manuscript; available in PMC: 2012 May 4.
Published in final edited form as: J Am Chem Soc. 2011 Apr 6;133(17):6681–6691. doi: 10.1021/ja111009s

Figure 2.

Figure 2

FT-IR spectra of CO-bound cyt c Ht-M61A at 25 °C (native protein), at 57 °C (overlapping bands of the native and unfolded protein), and 87 °C (unfolded protein) in 3 M GuHCl, 100 mM sodium phosphate, D2O, pD 7,. The black curves are the data. The dashed red curves are Gaussian fits to the data. In the middle panel, the spectrum was fit to the sum of two Gaussian bands. The blue (native) and green (unfolded) curves are the two Gaussians that result from the fit.