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Comparison between temperature dependent CD signal at 222 nm (normalized) the FT-IR band area ratio, RA. RA is the ratio of the area of the unfolded band to the sum of the areas of the native and unfolded bands. Both types of data give the appearance of two-state protein unfolding. The transition temperatures (Tm) are 56 ± 1 °C and 60 ± 1 °C for RA and CD, respectively.
