Table 2.

In silico analysis of CO1 mutations of data presented in Avtzis et al. (2008). Total number and relative amount of mutational patterns observed in a 1557 bp stretch of n=262 individuals is compared with expected values for authentic mtDNA.

	Total	Relative (%)	Expected value for mthNA a
Single base substitutions	125	100.0	-
1st codon position substitutions	15	12.0	14.9 ± 9.4% b
2nd codon position substitutions	2	1.6	4.5 ± 3.5% b
3rd codon position substitutions	108	86.4	80.6 ± 21% b
Nonsynonymous substitutions	13	10.4	7.47 ± 5.4% c
C › T substitutions	25	20.0	-
GC › GT substitutions	3	12.0 d	25 ± 14.0% e
Insertions	0	0	none f
Deletions	0	0	none f
Additional stop-codons	0	0	none f
Transitions (3rd codon position)	95	88.0 g	84.9% ± 18.1% h
Transversions (3rd codon position)	13	12.0 g	15.1 ± 7.6% h
Trasition-transversion ratio	7.31	-	-
GC content	-	34.6	28.66 ± 10.5% i

^a expected relative values as given in reference ± χ ² confidence interval at α=0.05 (Sachs 1999), ^b Blouin et al. (1998), ^c Shoemaker et al. (2004), data of Drosophila subquinaria, ^d percentage GC › GT substitutions of total C › T substitutions, ^e Bulmer (1986), Bensasson et al. (2001), ^f Zhang and Hewitt (1996), ^g percentage of total transitions/transversions on 3 ^rd codon position, ^h Tamura (1992), ⁱ Lin and Danforth (2004), data for CO1 genes