| 1. | Apical margin of clypeus strongly thickened | Osmia nigriventris (Zetterstedt) |
| – | Apical margin of clypeus more or less flat | 2 |
| 2. | Propodeum shiny, propodeal triangle nearly completely polished or at least along sides with shiny area. Body length at least 11 mm [xanthomelana species group] | 3 |
| – | Propodeum and propodeal triangle completely shagreened and dull; body length at most 10 mm [inermis species group] | 6 |
| 3. | T2 with impunctate apical margin medially rather broad (ca. length of F10); T4 polished (excluding the shagreened, impunctate apical margin) | 4 |
| – | T2 with impunctate apical margin narrower; T4 more or less shagreened throughout | 5 |
| 4. | Integument with weak metallic sheen; mandible with rather weak, nearly absent, carina separating third tooth from second and fourth | Osmia pamirensis Gussakovskij |
| – | Integument lacking metallic sheen; mandible with relatively strong carina separating third tooth from second and fourth | Osmia maritima Friese, Osmia ephippiata Smith, and Osmia thoracica Radoszkowski [see Table 1 for further comments] |
| 5. | Mandible with third tooth directed in same plane as second and fourth teeth, not set off from dorsal surface of mandible by carina; T2 with hairs medially on disc relatively long (ca. 640 micrometers), predominantly yellow-brown | Osmia xanthomelana (Kirby) |
| – | Mandible with third tooth directed slightly towards inner surface of mandible, set off from dorsal surface of mandible by carina extending from inner margins of second and fourth teeth; T2 with hairs medially on disc relatively short (ca. 400 micrometers), entirely black | Osmia alticola Benoist |
| 6. | Declining basal portion of T1 densely shagreened, only with a silky luster | 7 |
| – | Declining basal portion of T1 shining, at most superficially shagreened in small areas | 10 |
| 7. | Mandible with third tooth broad, not strongly separated from fourth tooth; forewing with veins cu-v and M intersecting vein M+Cu at the same place | 8 |
| – | Mandible with third tooth more triangular; forewing with cu-v intersecting vein M+Cu at a point distal to that of M | 9 |
| 8. | Mandible on its inferior inner margin with prominent, asymmetrically triangular tooth; third tooth nearly filling entire space between second and fourth teeth; clypeus in profile only moderately convex | Osmia inermis (Zetterstedt) and Osmia ishikawai Hirashima |
| – | Mandible on its inferior inner margin nearly straight, without tooth; third tooth separated from second by indentation nearly one-third of entire space between second and fourth teeth; clypeus in profile strongly convex | Osmia disjuncta Tkalců |
| 9. | Mandible with third tooth symmetrically triangular, broadly separated from both fourth and second teeth by acute-angled and broad indentation; mesepisternum with hairs predominantly blackish, only on its most anterior and uppermost part with narrow zone yellowish-brown; T2 with marginal zone not impressed, with punctures of disc basomedially finer, separated by up to three puncture diameters | Osmia svenssoni Tkalců |
| – | Mandible with third tooth asymmetrically triangular, separated from second tooth by acute-angled and broad indentation, from fourth by shallow and rounded indentation; mesepisternum with hairs uniformly white to yellowish-white, sometimes with few intermixed blackish hairs; T2 with marginal zone impressed on its entire width, with punctures of disc basomedially coarser, separated by up to two puncture diameters | Osmia steinmanni Müller |
| 10. | Distance between lateral ocellus and preoccipital margin two ocellar diameters; T2 and T3 with relatively well-defined, completely impunctate apical margin; integument with slight blue metallic sheen (especially on head and metasomal terga) | Osmia parietina Curtis |
| – | Distance between lateral ocellus and preoccipital margin three ocellar diameters; T2 and T3 with relatively broad apical margins with sparse, unevenly scattered punctures; integument lacking any metallic sheen | 11 |
| 11. | Galea with short, completely straight hairs; mesepisternum with white to pale yellow hairs; T2 with hairs in the center of disc shorter (ca. 400 micrometers); T1 only with hairs strongly yellow-brown | 12 |
| – | Galea with much longer, incurved setae; mesepisternum with blackish hairs posteroventrally; T2 with hairs in the center of disc longer (ca. 560 micrometers); T1 and T2 with hairs strongly yellow-brown (T2 at the apical edge usually with weak admixture of black hair) | Osmia pilicornis Smith |
| 12. | Clypeus in profile more convex, with punctures near base and in paraocular area relatively large and shallow (punctures basolaterally on clypeus distinctly larger than punctures basomedially on clypeus); forebasitarsal segment relatively long and thin (length ca. 3.5 times longer than width), length slightly longer than foretarsal segments two to five | Osmia uncinata Gerstäcker |
| – | Clypeus in profile less convex, with punctures near base and in paraocular area relatively small and deep (punctures basolaterally on clypeus the same size as those basomedially on clypeus); forebasitarsal segment relatively short and thick (length less than 3.0 times longer than width), length slightly shorter than foretarsal segments two to five | Osmia laticeps Thomson |