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. Author manuscript; available in PMC: 2011 Oct 22.
Published in final edited form as: Immunity. 2011 Apr 22;34(4):566–578. doi: 10.1016/j.immuni.2011.03.018

Figure 3. IL-10R ablation does not affect Foxp3 expression and Treg cell lineage stability.

Figure 3

(A) Flow cytometric analysis of Foxp3 and YFP expression on splenic CD4+ T cells in Foxp3CreIl10rafl/flR26Y and control littermate mice. (B) Expression of Foxp3 in flow cytometry-sorted CD4+YFP+ T cells from indicated mice. (C) Quantitative PCR (qPCR) analysis of Foxp3 and IL-10Rα expression in flow cytometry-sorted CD4+YFP+ T cells. The grey bar represents CD4+YFP- T cells. The results represent the mean+/-SD of relative expression values for the indicated genes relative to hypoxanthine-guanine phosphoribosyl transferase. Data are representative of three independent experiments.