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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Nat Med. 2010 Nov 21;16(12):1421–1428. doi: 10.1038/nm.2250

Figure 3.

Figure 3

Overexpression of S1pr1 in tumor cells promotes tumor progression and activates Stat3. (a) S1PR1 expression in tumor cells on tumorigenesis in mice. Insets in top panels: Western blotting showing increased expression of S1pr1 in MB49 tumor cells by either dsRNA or plasmids encoding S1PR1. Left, Tumor growth was measured at the indicated time points; means ± s.e.m., n = 6. Right, numbers of spontaneous lung metastatic nodules in mice with control and S1PR1-expressing MB49 tumors. N.D., not detected; means ± s.e.m., n = 6.*, P<0.1.***, P<0.001. (b) Effects of S1pr1 expression on Stat3 activity. Top panels: quantification of S1pr1 mRNA levels by real-time RT-PCR in tumors consisting of both tumor cells and stromal cells including immune cells; means ± s.d., n = 3. Lower left and middle panels: Western blotting showing S1pr1 and p-Stat3 protein levels in MB49 tumors. Lower right panel: quantification of S1pr1 and p-Stat3 protein levels by Western blotting in tumor cells transfected with control or S1pr1 siRNA. (c) Real-time RT-PCR showing RNA expression levels of several Stat3 target genes in MB49 tumors with or without elevated S1pr1 expression; means ± s.d., n = 3.