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. Author manuscript; available in PMC: 2012 May 10.
Published in final edited form as: J Am Chem Soc. 2011 Apr 13;133(18):6871–6873. doi: 10.1021/ja201384w

Figure 3.

Figure 3

(A) Variable-temperature MCD spectra of oxidized ΔnifB NifEN (0.67 mM) showing a decrease in spectral intensity with an increase in temperature, which arises from a paramagnetic ground state of the [4Fe4S] cluster in this protein. (B) Magnetization curves of oxidized ΔnifB NifEN (red) and the simulation of data (blue) assuming S = 2, D = 1 cm−1 and E/D = 0.05, with polarizations Mxy = 1.0, Mxz = 1.0 and Myz = 1.0. Curves were recorded at 1.58 K (far right), 4.2 K (middle) and 9.8 K (far left).