. Author manuscript; available in PMC: 2011 May 5.

Published in final edited form as: J Biol Chem. 2007 Mar 27;282(19):14186–14193. doi: 10.1074/jbc.M700827200

TABLE 1. Rates of PQ²⁺-induced H₂O₂ production in cellular fractions.

Equal protein amounts from each fraction were assayed for H₂O₂ production using a fluorometric method following the addition of 250 μM PQ²⁺. H₂O₂ production in mitochondrial fraction stimulated with malate plus glutamate as respiration substrates was considered 100%, and all values are expressed as a percentage of this (mean ± S.E., n = 3).

Fraction	Substrate
Fraction	With endogenous substrate	With succinate	With Mal/Pyr	With Mal/Glu
Homogenate	0.21 ± 0.02^a	4.52 ± 0.19^a	7.99 ± 0.34^a	5.55 ± 0.17^a
Cytosol	0.02 ± 0.003	0.06 ± 0.01	0.03 ± 0.01	0.05 ± 0.02
Mitochondria	0.98 ± 0.07^a,^b	81.48 ± 3.53^a,^b	143.83 ± 6.11^a,^b	100.00 ± 3.10^a,^b

Significantly different from cytosolic fraction (p < 0.05, one-way analysis of variance) grouped by respiration substrate.

Significantly different from homogenate fraction (p < 0.05, one-way analysis of variance) grouped by respiration substrate.

TABLE 1. Rates of PQ2+-induced H2O2 production in cellular fractions.

TABLE 1. Rates of PQ²⁺-induced H₂O₂ production in cellular fractions.