TABLE 2. PQ2+uptake by rat brain mitochondria.
Isolated mitochondria (Mito) were incubated in 250 μM PQ2+ in the presence of respiration substrates and inhibitors, and intramitochondrial PQ2+ concentrations were measured via HPLC. Values are presented as mean ± S.E., n = 3.
Condition | Intramitochondrial PQ2+ |
---|---|
μM | |
Mito + PQ2+ + buffer | 137.5 ± 50 |
Mito + PQ2+ + buffer + sonication | 20 ± 12.5 |
Mito + PQ2+ + buffer + succinate | 162.5 ± 75 |
Mito + PQ2+ + buffer + succinate + antimycin A | 225 ± 50 |
Mito + PQ2+ + buffer + succinate + FCCP | 200 ± 75 |