Figure 4.

Ac-EEED peptide reduces experimentally induced CNV lesion volume in a concentration-dependent manner. (A–E) Typical and representative z-stack compressions of confocal image captures of CNV lesions from mice receiving vehicle, scrambled peptide, or 20 ng, 200 ng, or 500 ng Ac-EEED peptide, respectively. Red fluorescence results from TRITC-conjugated R. communis agglutinin I that binds to endothelium. Scale bar, 100 μm. (F) Summary of the quantitative changes in CNV lesion volume resulting from increasing concentrations of Ac-EEED (n = 5 animals per condition). There was marked observable toxicity to the eyes with the highest dose tested. *P < 0.05 versus vehicle treatment; **P < 0.01 versus vehicle treatment.