Table 1.
List of Antibodies and Their Sources with the Applied Methods Used in the Study
| Antigen Detection | Primary Antibodies | Secondary Antibodies |
|---|---|---|
| Choleratoxin subunit B; immunoperoxidase | Goat anti-choleratoxin 1:20,000; List Biological Laboratories, Campbell, CA | Biotinylated rabbit anti-goat 1:200; Vector Laboratories, Burlingame, CA |
| Choleratoxin subunit B; fluorescence | Goat anti-choleratoxin 1:5 000; List Biological Laboratories | Cy3 donkey anti-goat 1:200; Jackson Immuno Research, West Grove, PA |
| Tetramethylrhodamine dextran; immunoperoxidase | Rabbit anti-tetramehylrhodamine dextran 1:6000; Invitrogen-molecular probes, Eugene, OR | Biotinylated goat anti-rabbit 1:200; Vector Laboratories |
| Tetramethylrhodamine dextran; fluorescence | Rabbit anti-tetramethylrhodamine dextran 1:2000; Invitrogen-molecular | Cy3 donkey anti-rabbit 1:200; Jackson ImmunoResearch |
| Snap 25; fluorescence | Mouse anti-snap-25 1:1000; Sternberger monoclonals Inc., Baltimore, MD | Alexa 488 donkey anti-mouse 1:200; Invitrogen-Molecular Probes |
| Synaptophysin; fluorescence | Mouse anti-synaptophysin 1:20; m0776, Dako, Glostrup, Denmark | Alexa 488 donkey anti-mouse 1:200; Invitrogen-Molecular Probes |
| Myosin heavy chain (slow); immunoperoxidase | Mouse anti-myosin heavy chain (slow) (MHCS) 1:100; Novocastra | Biotinylated horse anti-mouse 1:200; Vector Laboratories |
| Laboratories Ltd, Newcastle-upon-Tyne, UK | ||
| Tyrosine hydroxylase; fluorescence | Rabbit anti-tyrosine hydroxylase 1:100; ab152; Chemicon, Billerica, MA | Alexa 488 donkey anti-rabbit 1:200; Invitrogen-Molecular Probes |
| Choline acetyltransferase; fluorescence | Goat anti-choline acetyltransferase 1:50; ab144p; Chemicon | Alexa 488 donkey anti-goat 1:200; Invitrogen-Molecular Probes |