Figure 1.

In silico primer extension yields results comparable to those of conventional methods. The upper panel displays the frequency distribution of the 5′ends of transcripts of the human aldolase A gene as derived from DBTSS (9). The figure in the lower panel [reprinted from (11) with permission from Elsevier] summarizes the results of mRNA 5′end mapping experiments carried out by conventional techniques for the same gene. Note that in silico primer extension successfully identified all four promoter regions reported before.