Fig. 2.
Experimental measurements of protein dynamics using iFRAP. (A–C) Images from iFRAP experiments and sample curves for proteins GFP-Rho3, GFP-Rho1, and Pea2-GFP. Red lines encircle bleached areas, and yellow lines encircle areas of fluorescence measurements. Red arrows indicate the time of iFRAP bleaching. The raw fluorescence measurements were subjected to monoexponential curve fitting (solid red curves). (Scale bar: 2 μm.) (D) Dynamics (measured as iFRAP t1/2) of 11 proteins in the Signaling module. Boxes show the range of 1 SEM from the mean, represented by small squares. Vertical bars show the range of 1 SD from the mean. The horizontal line in the boxes represents the median. (E) Dynamics (measured as t1/2) of 18 proteins in the Transport module represented as described in D. Note that the vertical time scale (0–50 s) for the 15 proteins on the left is different from that (0–180 s) for the 3 proteins on the right.