Fig. 4.
Early progenitor cells in the fetal liver of E14.5 embryos. (A) Total number of cells for embryos of the indicated genotypes. (B) Representative flow cytometric profile of a Ccr9+/−;Ccr7+/−;Cxcr4+/− embryo to illustrate the identification of Lin−CD117+CD127+PIR− and Lin−CD117+CD127+PIR+ populations. Numbers refer to percentages of total cells. (C) Number of Lin−CD117+CD127+PIR− cells in the fetal liver of embryos with the indicated genotypes. (D) Number of Lin−CD117+CD127+PIR+ cells. (E) Total number of cells and number of DN2 + DN3 cells obtained after in vitro culture of 100 Lin−CD117+CD127+PIR+ cells for 8 d on OP9-DL1 stroma. The total number of embryos analyzed per genotype is indicated (n).