Fig. 3.

RecA mediated PIFE effect on ssDNA. (A) Schematic of RecA monomers forming a filament on single-strand DNA. (B) A representative single-molecule trace displaying RecA monomer binding and dissociation. PIFE levels correspond to M₀ to M₄ states. (C) Intensity histograms from the clustering analysis show a narrow single peak for DNA only (top) and appearance of distinct peaks corresponding to M₀ to M₄ states when RecA is added (bottom). (D) Plot of the average fold increase obtained from five independent measurements.