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. 2011 Apr 18;108(18):7351–7356. doi: 10.1073/pnas.1015686108

Fig. 2.

Fig. 2.

Targeting Zn2+ sensors to ER and Golgi in HeLa cells. (A) Fluorescence image of ER-ZapCY1. (B) Representative traces (n = 6 cells) for ER-ZapCY1. The FRET ratio decreased with 150 μM TPEN/10 μM thapsigargin and increased with addition of 5 μM pyrithione and 10 nM Zn2+. (C) Representative traces (n = 6 cells) for ER-ZifCY1. The FRET ratio did not change with TPEN but increased with 25 μM pyrithione, 0.001 mg/mL saponin, and 500 μM Zn2+. (D) Fluorescence image of Golgi-ZapCY1. (E) Representative traces (n = 4 cells) for Golgi-ZapCY1. The FRET ratio decreased with 150 μM TPEN and increased with addition of 5 μM pyrithione and 10 nM Zn2+. (F) Representative traces for Golgi-ZifCY2 (n = 7 cells). The FRET ratio did not change with TPEN but increased with addition of 25 μM pyrithione, 0.001 mg/mL saponin, and 500 μM Zn2+.