Figure 8. Effect of polymorphic LTR element insertions on transcription of the Trpc6 and Kcnh6 genes.
Quantitative RT-PCR of the Trpc6 (A) and Kcnh6 (B) genes using brain RNA from the indicated mouse strains. Green bars show the amount of transcripts detected by the primer set upstream the polymorphic IAP insertion, and blue bars show the amount of transcripts detected by the primer set flanking the location of the IAP insertion. Each bar represents the mean of at least 4 experiments ± standard deviation, which was first normalized to β-actin levels in the queried strain, and then represented relative to 5′ expression levels for each gene in B6 mice. The plus/minus sign shows the presence/absence of the IAP insertion in the corresponding mouse strain, respectively.