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. 2011 May 5;6(5):e18254. doi: 10.1371/journal.pone.0018254

Figure 3. 1-DE western blot analysis of NB cells treated or not with ATRA.

Figure 3

Representative western blot (of three independent experiments) with anti-phosphoserine (A) and anti-phosphotyrosine (B) antibodies. SJ-N-KP cells were treated with ATRA 10 µM for 30, 60 minutes, 3, 9, 24, 48 hours and 9 days. Control cells (c) were not treated. 15 µg of proteins were analysed on 10% SDS-polyacrylamide gel. Western blot analysis with anti-actin antibodies was performed on the same membranes and used for data normalization. Data (means ± SD) expressed as densitometric units are shown in the lower corner of panels A and B. Significance of the differences between controls and ATRA treated cells: p≤0.05.