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. 2011 May 5;6(5):e19297. doi: 10.1371/journal.pone.0019297

Figure 5. Interaction between HEN2 and HES1 in cells.

Figure 5

(A) Neuro2a cells were co-transfected with the indicated combinations of expression plasmids. Forty-eight hours after transfection, cells were lysed and immunoprecipitated with anti-FLAG (left panel) or with anti-Myc tag antibody (right panel) and the immunoprecipitates were analyzed by immunoblotting with anti-HES1 or with anti-FLAG antibody, respectively. Aliquots of cell lysates were subjected to immunoblotting with anti-HES1, anti-FLAG or with anti-Myc tag antibody. (B) In vitro pull-down assay. Radio-labeled FLAG-HEN2 was incubated with cell lysates prepared from Neuro2a cells transfected with Myc-HES1 expression plasmid. The reaction mixture was immunoprecipitated with normal rabbit serum (NRS) or with polyclonal anti-Myc tag antibody and separated by SDS-PAGE followed by autoradiography. 1/5 inputs were also shown.