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. 2011 May 5;6(5):e19407. doi: 10.1371/journal.pone.0019407

Figure 1. Schematic diagram of the construction of mc-oriP and control vectors and the generation of mc-oriP-IFNγ.

Figure 1

(A) Diagram of the minicircle vectors carrying the IFNγ gene or firefly luciferase gene under the control of the oriP promoter (left) or CMV promoter (right). (B) Flow chart of ФC31 integrase-mediated intramolecular recombination of p2ФC31-oriP-IFNγ. The resulting product is minicircle-oriP-IFNγ. Amp, ampicillin resistance gene; BAD, araBAD promoter; araC, araC repressor; attB, bacterial attachment site; attP, phage attachment site; attR, right hybrid sequence; I-SceIg, I-SceI gene; I-SceIs, I-SceI cutting site; L-arab., L-arabinose.