Figure 5. UV exposure and transcription inhibition potentiates the induction of HR in mammalian cells.

AA8 and irs1SF cells were treated with 20 µM DRB for 24 h, 10 J.m⁻² UV or both. (A) Confocal through focus maximum projection images showing RAD51 staining in red and DNA staining in blue. Bar 10 µm. (B) Quantification of RAD51 positive cells. Cells with more than 10 bright foci were considered positive. The means and S.E. (bars) of six experiments with 200–400 cells counted for each experiment are shown. Values marked with asterisks are significantly different from control in T -test (**P<0.01, ***P<0.001).