Figure 4. PTEN inhibits migration of tumor cells.
(A) A wound healing assay was performed on HepG2 cells (1×106, 6-cm plate) transfected with random siRNA, PTEN siRNA, pcDNA3-29a, and both pcDNA3-PTEN/pcDNA3-29a, respectively. One representative experiment is shown. Black arrows indicate the wound edge. (B) Modified Boyden's chamber assays showed that enhanced cell migration induced by miR-29a was reversed by the overexpression of PTEN in HepG2 cells. A histogram shows the relative cell number of five randomly selected fields. (C) The expression of PTEN was detected by western blot analysis in above transfected HepG2 cells. Statistically significant differences are indicated: *P<.05, **P<.01, Student's t test.