Figure 1. Strategies used to amplify the near genome-wide HCV genome from 9 participants.

The top bar illustrates the HCV genomic organization with 10 encoded proteins (Core to NS5B) flanked by the 5′ and 3′ UTRs. Lines below represent the overlapping fragments amplified by reverse transcription PCR for each participant (PCR primers listed in Table S1). The nucleotide numbering is based on the sequence of HCV H77 strain (Genbank assession no. NC_004102).