Figure 7. Virus requirements for basolateral surface entry.

Transfer infection was performed with B cells infected for 24 h with recombinant EBV carrying deletions (Δ) for the glycoproteins gp42 and gp85, plus the WT virus without reconstituted gp110 (gp110 low), and their respective complemented (restored) glycoprotein counterparts. The B cells were co-cultured with polarized primary tonsillar epithelial cells at the basolateral surface for 1 hour, washed off and the epithelial cells trypsinised from the transwell and re-plated. Epithelial infection was monitored by flow cytometric analysis of GFP expression after 24 hours and plotted as % infection. Due to the inherent variability in infection between donor epithelial cells, assays were performed on triplicates of the same donor cells.