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. 2011 May 5;7(5):e1002065. doi: 10.1371/journal.pgen.1002065

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Hao et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) MudPIT analysis on proteins co-immunoprecipitated with FLAG-tagged Dnttip1 or ZNF541 from HEK293 cells. dNASF (distributed normalized spectral abundance factors) indicates the relative abundance of proteins. Results are from 3 independent biological samples. (Mean±SD). (B) Co-immunoprecipitation of FLAG-tagged HDAC1 with HA-tagged ZNF541 or Dnttip1 after co-expression in HEK293 cells. (C) Co-immunoprecipitation of FLAG-tagged HDA-2 with HA-tagged SAEG-2 after co-expression in Drosophila S2 cells. (D) Representative images of bacterial lawns on which a single animal was placed for 18 hours at 20°C. Areas covered by worm tracks (enclosed by a white line) were measured as an indicator of foraging behavior of individual animals. Excessive dwelling behavior by egl-4(gf) animals resulted in the smallest area in which worm tracks were found. (E) Quantitation of bacterial lawn area that was explored by individual animals of indicated genotypes. Number of animals tested: n = 60 (wild type and egl-4(gf)); n = 30 (all other genotypes). (Mean±SD; pair-wise t-test, *, p<0.05)