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. 2011 May 5;7(5):e1002065. doi: 10.1371/journal.pgen.1002065

Figure 5. Y45F10C.2 is an EGL-4 activity responsive gene that regulates egg laying.

Figure 5

(A) Gene expression profiling using Affymetrix microarray discovered 60 genes that were repressed in egl-4(gf) and activated in egl-4(lf) animals when compared with wild-type animals, using a cut-off of 1.5-fold change, p<0.05. (B) Top ten differentially expressed genes in egl-4(gf) versus egl-4(lf) animals. Fold change based on microarray analysis of three independent samples of each genotype is shown. (C) Real-time PCR analysis of Y45F10C.2 gene expression in wild-type and egl-4 mutant animals at young adult stage. At least 2 independent RNA samples were analyzed for each genotype and the expression level in wild-type animals (WT) was set as 1. (D) Number of eggs retained in uterus in transgenic animals over-expressing wild-type or mutant Y45F10C.2 transgenes. Number of animals tested: n = 19 (WT and Ex[Y45F10C.2(C36A)] line A); n = 18 (Is[Y45F10C.2] and Ex[Y45F10C.2(C36A)] line B). (E) Representative image of a 2-day old adult animal carrying a Y45F10C.2p::Y45F10C.2::SL2::GFP-PEST transgene. Overexpression of Y45F10C.2 caused retention of eggs in the uterus. (F) Representative image of a 2-day old adult animal carrying a Y45F10C.2p::Y45F10C.2(Cys36Ala)::SL2::GFP transgene. Overexpression of mutant Y45F10C.2 did not affect egg-laying. (G) Representative image of a 2-day old adult egl-4(gf) animal carrying a Y45F10C.2p::Y45F10C.2::SL2::GFP-PEST transgene. Expression of the transgene was repressed.