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. 2011 May 5;7(5):e1002032. doi: 10.1371/journal.ppat.1002032

Figure 8. Infected erythrocyte binding to other candidate cytoadhesion receptors.

Figure 8

Parasites in the panel were assessed for binding to transfected CHO745 cell lines or recombinant proteins. (A) Infected erythrocyte binding to CHO cells expressing chondroitin sulfate A (CHO-K1), E-selectin (CHO745-ELAM-1), or vascular leukocyte adhesion molecule 1 (CHO745-VCAM-1). (B) Infected erythrocyte binding to immobilized chondroitin sulfate A (CSA) and the recombinant proteins TSP-1, HABP1, CD31, endoglin, and fractalkine. All proteins and CSA were immobilized on polystyrene substrate at 50 µg/mL. Infected erythrocyte binding to transfected cell lines were corrected by subtracting background binding to untransfected CHO-745 cells. Binding to recombinant proteins was corrected by substracting binding to bovine serum albumin, which was employed as the blocking agent in all assays. An arbitrary threshold for positive binding (grey dashed line) was calculated as the mean level of infected erythrocyte binding plus two standard deviations either to untransfected CHO745 cells or to spots containing 2% bovine serum albumin, respectively. Error bars represent the range of binding between two replicate experiments.