Skip to main content
. 2011 May 5;7(5):e1002022. doi: 10.1371/journal.ppat.1002022

Figure 4. Natural sequence variation in the Y-Sat siRNA-targeted region prevents sRNA-directed silencing of CHLI and hence development of yellowing symptoms upon CMV Y-Sat infection.

Figure 4

(A), Typical phenotypes upon CMV Y-Sat infection of three disease susceptible, N. glutinosa, N. benthamiana and N. tabacum, and two disease-resistant species, N. clevelandii and N. debneyi. (B), Sequencing trace files showing the presence of single nucleotide changes in the CHLI mRNA from the two disease-resistant species. The asterisk (*) indicates the variant nucleotide position, with the number of individual RT-PCR clones containing the specific nucleotide variant given above the corresponding trace file peak. (C), CHLI mRNA from N. clevelandii and N. debneyi is resistant to Y-Sat-induced silencing. The ‘+’ and ‘-’ symbols indicate samples from plants infected (+) or uninfected (−) with CMV Y-Sat.