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. Author manuscript; available in PMC: 2012 Apr 29.
Published in final edited form as: Cell. 2011 Apr 14;145(3):423–434. doi: 10.1016/j.cell.2011.03.022

Figure 6. TET1 and AID Regulate Endogenous 5hmC Levels and Promote DNA Demethylation in the Adult Mouse Brain.

Figure 6

(A and B) Effects of AAV-mediated overexpression of control YFP, TET1, TET1m (A), and AID (B) on the endogenous 5hmC levels as measured by immunoblotting (left) and ELISA (right). Engineered AAV viruses were stereotaxically injected into the dentate gyrus of adult mice and dentate gyrus tissue was micro-dissected one week later for analysis. Values represent mean ± SEM (n = 3 animals for each condition; **: P < 0.01; Student’s t-test).

(C and D) Effects of YFP, TET1, TET1m and AID overexpression on methylation levels of Bdnf IX (C), Fgf1B and Fgf1G (D) alternative promoters in the adult dentate gyrus. Shown on top are diagrams of promoters and bars indicate regions for bisulfite sequencing analysis. Values represent mean ± SEM (n = 3 animals for each condition; **: P < 0.01; *: P < 0.05; Student’s t-test).

(E) Effects of YFP, TET1, TET1m and AID overexpression on specific isoform and total expression levels of Fgf1 and Bdnf in the adult dentate gyrus. Same groups of animals as in (C and D) were examined. Values represent mean ± SEM (**: P < 0.01; *: P < 0.05; Student’s t-test).