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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Mol Microbiol. 2011 Jan 24;79(6):1557–1573. doi: 10.1111/j.1365-2958.2011.07546.x

Figure 7.

Figure 7

(A) Growth curves of the meningococcal strains in standard GC broth. (B) Heme staining of the wild type, dsbA2A1 and dsbA2A1D strains. The assignment of each band is based on the previous report (Li et al., 2010). (C) Natural competence of the wild-type NMB and its mutants. Bacteria were transformed with chromosomal DNA from a CmR derivative of NMB, and cfu counts were determined on selective and non-selective plates. Transformation frequencies were calculated by dividing the cfu numbers of selective plates by the cfu numbers of non-selective plates. The frequency of the mutant was normalized to that of the wild type, which was set as 100%. Data are the means ± standard deviations of two assays done in duplicate. (D) PilQ levels determined by Western blots. Equal amounts of whole cell lysates (8 μg) were resolved on 10% SDS-PAGE gels and transferred to PVDF membranes. The top half of the membrane was probed with PilQ antisera (Tonjum et al., 1998), while the lower half was probed with Opa antisera (Takahashi et al., 2008) for loading controls.